Adipokinetic hormones (AKHs) of sphingid Lepidoptera, including the identification of a second M. sexta AKH.
Identifieur interne : 002C12 ( Main/Exploration ); précédent : 002C11; suivant : 002C13Adipokinetic hormones (AKHs) of sphingid Lepidoptera, including the identification of a second M. sexta AKH.
Auteurs : Robert J. Weaver [Royaume-Uni] ; Heather G. Marco ; Petr Simek ; Neil Audsley ; Kevin D. Clark ; Gerd G DeSource :
- Peptides [ 1873-5169 ] ; 2012.
Descripteurs français
- KwdFr :
- Acide pidolique (analogues et dérivés), Acide pidolique (composition chimique), Acide pidolique (métabolisme), Animaux (MeSH), Hormones des insectes (composition chimique), Hormones des insectes (métabolisme), Lepidoptera (métabolisme), Manduca (métabolisme), Oligopeptides (composition chimique), Oligopeptides (métabolisme), Spectrométrie de masse ESI (MeSH), Spectrométrie de masse MALDI (MeSH), Spectrométrie de masse en tandem (MeSH).
- MESH :
- analogues et dérivés : Acide pidolique.
- composition chimique : Acide pidolique, Hormones des insectes, Oligopeptides.
- métabolisme : Acide pidolique, Hormones des insectes, Lepidoptera, Manduca, Oligopeptides.
- Animaux, Spectrométrie de masse ESI, Spectrométrie de masse MALDI, Spectrométrie de masse en tandem.
English descriptors
- KwdEn :
- Animals (MeSH), Insect Hormones (chemistry), Insect Hormones (metabolism), Lepidoptera (metabolism), Manduca (metabolism), Oligopeptides (chemistry), Oligopeptides (metabolism), Pyrrolidonecarboxylic Acid (analogs & derivatives), Pyrrolidonecarboxylic Acid (chemistry), Pyrrolidonecarboxylic Acid (metabolism), Spectrometry, Mass, Electrospray Ionization (MeSH), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH), Tandem Mass Spectrometry (MeSH).
- MESH :
- chemical , analogs & derivatives : Pyrrolidonecarboxylic Acid.
- chemical , chemistry : Insect Hormones, Oligopeptides, Pyrrolidonecarboxylic Acid.
- chemical , metabolism : Insect Hormones, Oligopeptides, Pyrrolidonecarboxylic Acid.
- metabolism : Lepidoptera, Manduca.
- Animals, Spectrometry, Mass, Electrospray Ionization, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry.
Abstract
The adipokinetic hormones (AKHs) from the corpora cardiaca (CC) of representative species from all three subfamilies of the Sphingidae (hawkmoths) were investigated using matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) and liquid chromatography electrospray ion trap mass spectrometry (LC-ESI MS), including a re-examination of the AKH complement of the tobacco hawkmoth, Manduca sexta. In addition to larvae and adults of M. sexta (subfamily: Sphinginae), adults from the following subfamilies were examined: Macroglossinae (large elephant hawkmoth, Deilephila elpenor), Smerinthinae (poplar hawkmoth, Laothoe populi and eyed hawkmoth, Smerinthus ocellata), and Sphinginae (death's head hawkmoth, Acherontia atropos). All moths are shown to have the nonapeptide Manse-AKH (pELTFTSSWGamide) [corrected] in their CC, together with a second AKH, which, on the basis of mass ions ([M+Na](+), [M+K](+)) and partial sequence analysis is identical in all species examined. The structure of this AKH was extracted from the CC [corrected] of adult M. sexta and shown, by ESI-collision-induced dissociation (CID) tandem mass spectrometry (MS/MS), to be a novel decapeptide AKH with a sequence of pELTFSSWGQamide. [corrected]. The new peptide has been code named Manse-AKH-II. Sequence confirmation was obtained from identical MS studies with synthetic Manse-AKH-II and with the native peptide. Manse-AKH-II has significant lipid-mobilizing activity when injected at low dose (5pmol) into newly emerged adult M. sexta. The potential implications of a second AKH, in M. sexta in particular, are discussed in relation to putative receptor(s).
DOI: 10.1016/j.peptides.2012.01.009
PubMed: 22285789
Affiliations:
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Weaver</name><affiliation wicri:level="1"><nlm:affiliation>The Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, UK. robert.weaver@fera.gsi.gov.uk</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Food and Environment Research Agency, Sand Hutton, York YO41 1LZ</wicri:regionArea><wicri:noRegion>York YO41 1LZ</wicri:noRegion></affiliation></author><author><name sortKey="Marco, Heather G" sort="Marco, Heather G" uniqKey="Marco H" first="Heather G" last="Marco">Heather G. Marco</name></author><author><name sortKey="Simek, Petr" sort="Simek, Petr" uniqKey="Simek P" first="Petr" last="Simek">Petr Simek</name></author><author><name sortKey="Audsley, Neil" sort="Audsley, Neil" uniqKey="Audsley N" first="Neil" last="Audsley">Neil Audsley</name></author><author><name sortKey="Clark, Kevin D" sort="Clark, Kevin D" uniqKey="Clark K" first="Kevin D" last="Clark">Kevin D. Clark</name></author><author><name sortKey="G De, Gerd" sort="G De, Gerd" uniqKey="G De G" first="Gerd" last="G De">Gerd G De</name></author></analytic><series><title level="j">Peptides</title><idno type="eISSN">1873-5169</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Insect Hormones (chemistry)</term><term>Insect Hormones (metabolism)</term><term>Lepidoptera (metabolism)</term><term>Manduca (metabolism)</term><term>Oligopeptides (chemistry)</term><term>Oligopeptides (metabolism)</term><term>Pyrrolidonecarboxylic Acid (analogs & derivatives)</term><term>Pyrrolidonecarboxylic Acid (chemistry)</term><term>Pyrrolidonecarboxylic Acid (metabolism)</term><term>Spectrometry, Mass, Electrospray Ionization (MeSH)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH)</term><term>Tandem Mass Spectrometry (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide pidolique (analogues et dérivés)</term><term>Acide pidolique (composition chimique)</term><term>Acide pidolique (métabolisme)</term><term>Animaux (MeSH)</term><term>Hormones des insectes (composition chimique)</term><term>Hormones des insectes (métabolisme)</term><term>Lepidoptera (métabolisme)</term><term>Manduca (métabolisme)</term><term>Oligopeptides (composition chimique)</term><term>Oligopeptides (métabolisme)</term><term>Spectrométrie de masse ESI (MeSH)</term><term>Spectrométrie de masse MALDI (MeSH)</term><term>Spectrométrie de masse en tandem (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Pyrrolidonecarboxylic Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Insect Hormones</term><term>Oligopeptides</term><term>Pyrrolidonecarboxylic Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Insect Hormones</term><term>Oligopeptides</term><term>Pyrrolidonecarboxylic Acid</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Acide pidolique</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Acide pidolique</term><term>Hormones des insectes</term><term>Oligopeptides</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lepidoptera</term><term>Manduca</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide pidolique</term><term>Hormones des insectes</term><term>Lepidoptera</term><term>Manduca</term><term>Oligopeptides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Spectrometry, Mass, Electrospray Ionization</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Spectrométrie de masse ESI</term><term>Spectrométrie de masse MALDI</term><term>Spectrométrie de masse en tandem</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The adipokinetic hormones (AKHs) from the corpora cardiaca (CC) of representative species from all three subfamilies of the Sphingidae (hawkmoths) were investigated using matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) and liquid chromatography electrospray ion trap mass spectrometry (LC-ESI MS), including a re-examination of the AKH complement of the tobacco hawkmoth, Manduca sexta. In addition to larvae and adults of M. sexta (subfamily: Sphinginae), adults from the following subfamilies were examined: Macroglossinae (large elephant hawkmoth, Deilephila elpenor), Smerinthinae (poplar hawkmoth, Laothoe populi and eyed hawkmoth, Smerinthus ocellata), and Sphinginae (death's head hawkmoth, Acherontia atropos). All moths are shown to have the nonapeptide Manse-AKH (pELTFTSSWGamide) [corrected] in their CC, together with a second AKH, which, on the basis of mass ions ([M+Na](+), [M+K](+)) and partial sequence analysis is identical in all species examined. The structure of this AKH was extracted from the CC [corrected] of adult M. sexta and shown, by ESI-collision-induced dissociation (CID) tandem mass spectrometry (MS/MS), to be a novel decapeptide AKH with a sequence of pELTFSSWGQamide. [corrected]. The new peptide has been code named Manse-AKH-II. Sequence confirmation was obtained from identical MS studies with synthetic Manse-AKH-II and with the native peptide. Manse-AKH-II has significant lipid-mobilizing activity when injected at low dose (5pmol) into newly emerged adult M. sexta. The potential implications of a second AKH, in M. sexta in particular, are discussed in relation to putative receptor(s).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22285789</PMID><DateCompleted><Year>2012</Year><Month>07</Month><Day>12</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-5169</ISSN><JournalIssue CitedMedium="Internet"><Volume>34</Volume><Issue>1</Issue><PubDate><Year>2012</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Peptides</Title><ISOAbbreviation>Peptides</ISOAbbreviation></Journal><ArticleTitle>Adipokinetic hormones (AKHs) of sphingid Lepidoptera, including the identification of a second M. sexta AKH.</ArticleTitle><Pagination><MedlinePgn>44-50</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.peptides.2012.01.009</ELocationID><Abstract><AbstractText>The adipokinetic hormones (AKHs) from the corpora cardiaca (CC) of representative species from all three subfamilies of the Sphingidae (hawkmoths) were investigated using matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) and liquid chromatography electrospray ion trap mass spectrometry (LC-ESI MS), including a re-examination of the AKH complement of the tobacco hawkmoth, Manduca sexta. In addition to larvae and adults of M. sexta (subfamily: Sphinginae), adults from the following subfamilies were examined: Macroglossinae (large elephant hawkmoth, Deilephila elpenor), Smerinthinae (poplar hawkmoth, Laothoe populi and eyed hawkmoth, Smerinthus ocellata), and Sphinginae (death's head hawkmoth, Acherontia atropos). All moths are shown to have the nonapeptide Manse-AKH (pELTFTSSWGamide) [corrected] in their CC, together with a second AKH, which, on the basis of mass ions ([M+Na](+), [M+K](+)) and partial sequence analysis is identical in all species examined. The structure of this AKH was extracted from the CC [corrected] of adult M. sexta and shown, by ESI-collision-induced dissociation (CID) tandem mass spectrometry (MS/MS), to be a novel decapeptide AKH with a sequence of pELTFSSWGQamide. [corrected]. The new peptide has been code named Manse-AKH-II. Sequence confirmation was obtained from identical MS studies with synthetic Manse-AKH-II and with the native peptide. Manse-AKH-II has significant lipid-mobilizing activity when injected at low dose (5pmol) into newly emerged adult M. sexta. The potential implications of a second AKH, in M. sexta in particular, are discussed in relation to putative receptor(s).</AbstractText><CopyrightInformation>Crown Copyright © 2012. Published by Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Weaver</LastName><ForeName>Robert J</ForeName><Initials>RJ</Initials><AffiliationInfo><Affiliation>The Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, UK. robert.weaver@fera.gsi.gov.uk</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marco</LastName><ForeName>Heather G</ForeName><Initials>HG</Initials></Author><Author ValidYN="Y"><LastName>Simek</LastName><ForeName>Petr</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Audsley</LastName><ForeName>Neil</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Clark</LastName><ForeName>Kevin D</ForeName><Initials>KD</Initials></Author><Author ValidYN="Y"><LastName>Gäde</LastName><ForeName>Gerd</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>01</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Peptides</MedlineTA><NlmUniqueID>8008690</NlmUniqueID><ISSNLinking>0196-9781</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007301">Insect Hormones</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009842">Oligopeptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C044833">adipokinetic hormone</NameOfSubstance></Chemical><Chemical><RegistryNumber>SZB83O1W42</RegistryNumber><NameOfSubstance UI="D011761">Pyrrolidonecarboxylic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>Peptides. 2012 Dec;38(2):523</RefSource></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007301" MajorTopicYN="N">Insect Hormones</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007915" MajorTopicYN="N">Lepidoptera</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018402" MajorTopicYN="N">Manduca</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009842" MajorTopicYN="N">Oligopeptides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011761" MajorTopicYN="N">Pyrrolidonecarboxylic Acid</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="Y">analogs & derivatives</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021241" MajorTopicYN="N">Spectrometry, Mass, Electrospray Ionization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019032" MajorTopicYN="N">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053719" MajorTopicYN="N">Tandem Mass Spectrometry</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>11</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>01</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>01</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>1</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>1</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>7</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22285789</ArticleId><ArticleId IdType="pii">S0196-9781(12)00030-7</ArticleId><ArticleId IdType="doi">10.1016/j.peptides.2012.01.009</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><noCountry><name sortKey="Audsley, Neil" sort="Audsley, Neil" uniqKey="Audsley N" first="Neil" last="Audsley">Neil Audsley</name><name sortKey="Clark, Kevin D" sort="Clark, Kevin D" uniqKey="Clark K" first="Kevin D" last="Clark">Kevin D. Clark</name><name sortKey="G De, Gerd" sort="G De, Gerd" uniqKey="G De G" first="Gerd" last="G De">Gerd G De</name><name sortKey="Marco, Heather G" sort="Marco, Heather G" uniqKey="Marco H" first="Heather G" last="Marco">Heather G. Marco</name><name sortKey="Simek, Petr" sort="Simek, Petr" uniqKey="Simek P" first="Petr" last="Simek">Petr Simek</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Weaver, Robert J" sort="Weaver, Robert J" uniqKey="Weaver R" first="Robert J" last="Weaver">Robert J. Weaver</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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